Best seeds for your targets
Compound screening
Target identification

Advanced Affinity Selection Mass Spectrometry for Membrane Proteins

Our cutting-edge technology is a breakthrough in Affinity Selection Mass Spectrometry (ASMS), extending its application from soluble proteins to membrane proteins without the need for solubilization. This high-throughput method is versatile, accommodating any target type, and streamlines the screening process. Uniquely, our approach can be applied to orphan receptors, ion channels, transporters, and target RNAs, even those with unknown biological activity.
By utilizing a single, streamlined method across all target types, we can obtain highly specific binders for each target. Our extensive screening campaigns have built a robust binder collection, including specific binders for numerous GPCRs, SLC transporters, and ion channels. Our technology excels in identifying ligands for protein degraders as silent binders.

Impressive Achievements
To date, we have successfully identified binders for:
• 200 GPCRs, including orphan GPCRs
• 150 SLC transporters
Explore the potential of our advanced ASMS technology to revolutionize your research and drug discovery projects.

NEWS

2024-09-19 We will be exhibiting at JHVS (BIOJAPAN) from October 9th to 11th.
10月9日から11日に開催されるJHVS（BIOJAPAN）に出展します。
2024/04/04 結合タンパク質スクリーニングの化合物受付を開始しました。

Binder Collection (database)

  • Binder database for SLC transporter We have conducted screenings on several SLC transporters and obtained binding compounds for 154 of them. SLC6A9 is one of these transporters. The binders were tested for their ability to inhibit Glycine uptake in cells. In the graph, the vertical axis represents binding, while the horizontal axis shows the inhibition of Glycine uptake in cells. A very clear correlation is observed.
  • Binder database for GPCR We have conducted screenings on several GPCRs and obtained binding compounds for 197 of them. GPR87 is one of these GPCRs. The binders obtained were then tested using a Ca influx assay. In the graph, the vertical axis represents binding, while the horizontal axis shows the EC50 values from the Ca influx assay. We identified one compound as an antagonist, and the rest were agonists.
Target class No. of targets with binders
SLC transporter 154 SLC transporters
Amino acid: 32 (SLC1, SLC3, SLC6, SLC7, SLC16, SLC17, SLC18, SLC26, SLC32, SLC36, SLC38)
Metal ion: 8 (SLC11, SLC30, SLC31, SLC39, SLC40)
Sugar: 9 (SLC2, SLC5, SLC37, SLC45)
Others: 105
GPCR 197 GPCRs
[Class A]
orphan: 21, peptide: 35, lipid: 28
[Class B]
peptide: 5, adhesion: 15
[Class C]: 8
Others: 85

We are implementing a strategy of generating hits for each target class and selecting promising pipelines from them.
We are constructing binder collections using our protein library and compound library. We have already had binders of some SLC transporters and GPCRs as pipelines in discovery stage.

Target identification

For compounds with unknown target proteins, we use our proprietary technology to screen for binding interactions with proteins expressed from our library of approximately 17,000 cDNA clones.